• Biosphere 2 Mission One, September 26, 1991 - September 26, 1993, Organization
• Biosphere 2 Transition One, September 26, 1993 - March 6, 1994, Organization
• Biosphere 2 Mission Two, March 6, 1994 - April 1, 1994, Organization

• Margret Augustine: CEO, reviewed all aspects of Mission One and she and Allen had to approve all decisons.
• John Allen: Vice President Biospheric Development (divisions of research, development, engineering, environmental applications, analytics)
• Abigail Alling: Assistant Vice President of Biospheric Development
• William Dempster: Director of Engineering
• Norberto Alvarez-Romo: Head of Mission Control (including the review of all medical and health situations with the biospherians and consulting Doctors)

• Co-Captains: Sally Silverstone and Mark Van Thillo
• Director of Research: Abigail Alling
• Chief Medical Officer: Roy Walford
• Communications Officer : Mark Nelson

Area 1: Overall Agriculture - Sally Silverstone
Manager of Biosphere 2 Food Systems - Sally Silverstone
Manager of Field Agriculture Crops - Jane Poynter

Area 2: Overall Technical systems - Mark Van Thillo
Analytical Systems - Taber MacCallum

Area 3 : Medical Systems: Roy Walford
Assistant - Taber MacCallum

Area 4: Marine Biomes (Ocean, Marsh) - Abigail Alling

Area 5: Terrestrial Biomes (Rain forest, Desert, Savannah) - Linda Leigh
Terrestrial Biomes Assistant - Mark Nelson
Stream Systems - Abigail Alling

Area 6: Waste Recycling Systems - Mark Nelson

• Margret Augustine: CEO, reviewed all aspects of Mission Two and she, Allen and Alling had to approve all decisions.
• John Allen: Vice President Biospheric Development (divisions of research, development, engineering, environmental applications, analytics)
• Abigail Alling: Assistant Vice President of Biospheric Development
• William Dempster: Director of Engineering
• Norberto Alvarez-Romo: Head of Mission Control (including the review of all medical and health situations with the biospherians and consulting Doctors)
• Sally Silverstone: Mission coordinator

Mark Van Thillo, Head of Technical Systems, all technical upgrades, maintenance and technical Biosphere 2 tasks

Sally Silverstone, Head of Crew Training, in charge of training the crew for Mission Two and coordination with Mission Control

• Margret Augustine: CEO, reviewed all aspects of Mission Two and she and Allen had to approve all decisions.
• John Allen: Vice President Biospheric Development (divisions of research, development, engineering, environmental applications, analytics)
• Abigail Alling: Assistant Vice President of Biospheric Development
• William Dempster: Director of Engineering
• Norberto Alvarez-Romo: Head of Mission Control (including the review of all medical and health situations with the biospherians and consulting Doctors)

General Operations Coordinator:
John Druitt
Supervisor: Sally Silverstone

Watch Emergency Operations Officer:
Pascal Maslin
Supervisor: Mark Van Thillo

Biosphere 2 Research Coordinator:
Matt Finn
Supervisor: Abigail Alling

Electro-mechanical Systems Manager:
Pascal Maslin
Supervisor: Mark Van Thillo

Agriculture and Food Systems Manager
John Druitt
Supervisor: Sally Silverstone

Marine Systems Manager:
Matt Finn
Supervisor: Abigail Alling

Terrestrial Wilderness Systems Manager
Charlotte Godfrey
Supervisor: Mark Nelson

Assistant Agriculture Systems Manager:
Tilak Ram Mahato
Supervisor: John Druitt

Animal Systems Manager
Rodrigo Fernandez del Valle
Supervisor: John Druitt

Monitoring Systems manager
Rodrigo Fernandez del Valle
Supervisors: Gary Hudman, Mark Van Thillo

Safety Manager
Rodrigo Fernandez del Valle
Supervisor: Mark Van Thillo